TABLE 1.
Strain or plasmid | Genotype | Phenotype or characteristic | Reference |
---|---|---|---|
A. vinelandii | |||
MV17 | nfrX16::Tn5a | Nif sup? (fast growing) | 16 |
MV71 | glnD1::Ω glnY1 | Nif GSc | 5 |
S. meliloti | |||
GMI708 | Strain 2011 derivative | Rifr | 3 |
GMI3105 | glnA1 | GSI unadenylylatable | 1 |
Plasmids | |||
pBluescriptII KS+ | Ampr; cloning vector | Stratagene | |
pGEM-T Easy | Ampr; cloning vector self-ligated at EcoRV | Promega | |
pHP45Ω | Ampr Spr; carries Ω interposon | 14 | |
pJRD215 | IncQ broad-host-range vector; Kanr Smr | 7 | |
pJQ200KS | Gmr; sacB mobilizable cloning vector | 15 | |
pLAFR3 | IncP cosmid; Tetr | 13 | |
pPR601 | Derivative of pTA30 with HindIII fragment deleted | This study | |
pPR602 | pBluescript KS+ with 5.2-kb PstI fragment | This study | |
pPR604 | pBluescript KS+ with 13-kb HindIII fragment | This study | |
pPR608 | pJRD215 with 13-kb HindIII fragment | This study | |
pPR611 | pJRD215 with 5.2-kb ClaI/SacI fragment; glnD+ | This study | |
pPR612 | pGEM-T Easy carrying 5.2-kb PstI fragment | This study | |
pPR613 | pPR612 with Ω inserted at HindIII; glnD1::Ω | This study | |
pPR614 | pJQ200KS carrying glnD1::Ω | This study | |
pPR615 | pBluescriptII KS+ with 3.6-kb HindIII/SacI fragment | This study | |
pPR617 | pPR615 with Ω inserted at PstI blunt end; mviN1::Ω | This study | |
pPR618 | pJQ200KS with mviN1::Ω | This study | |
pTA30 | Library clone with mutS glnD mviN | This study |
nfrX was the previous designation for A. vinelandii glnD (6).