TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Phenotype or characteristic | Reference |
|---|---|---|---|
| A. vinelandii | |||
| MV17 | nfrX16::Tn5a | Nif sup? (fast growing) | 16 |
| MV71 | glnD1::Ω glnY1 | Nif GSc | 5 |
| S. meliloti | |||
| GMI708 | Strain 2011 derivative | Rifr | 3 |
| GMI3105 | glnA1 | GSI unadenylylatable | 1 |
| Plasmids | |||
| pBluescriptII KS+ | Ampr; cloning vector | Stratagene | |
| pGEM-T Easy | Ampr; cloning vector self-ligated at EcoRV | Promega | |
| pHP45Ω | Ampr Spr; carries Ω interposon | 14 | |
| pJRD215 | IncQ broad-host-range vector; Kanr Smr | 7 | |
| pJQ200KS | Gmr; sacB mobilizable cloning vector | 15 | |
| pLAFR3 | IncP cosmid; Tetr | 13 | |
| pPR601 | Derivative of pTA30 with HindIII fragment deleted | This study | |
| pPR602 | pBluescript KS+ with 5.2-kb PstI fragment | This study | |
| pPR604 | pBluescript KS+ with 13-kb HindIII fragment | This study | |
| pPR608 | pJRD215 with 13-kb HindIII fragment | This study | |
| pPR611 | pJRD215 with 5.2-kb ClaI/SacI fragment; glnD+ | This study | |
| pPR612 | pGEM-T Easy carrying 5.2-kb PstI fragment | This study | |
| pPR613 | pPR612 with Ω inserted at HindIII; glnD1::Ω | This study | |
| pPR614 | pJQ200KS carrying glnD1::Ω | This study | |
| pPR615 | pBluescriptII KS+ with 3.6-kb HindIII/SacI fragment | This study | |
| pPR617 | pPR615 with Ω inserted at PstI blunt end; mviN1::Ω | This study | |
| pPR618 | pJQ200KS with mviN1::Ω | This study | |
| pTA30 | Library clone with mutS glnD mviN | This study |
a
nfrX was the previous designation for A. vinelandii glnD (6).