a, Connection of the porcine body to the OrganEx perfusion system (or ECMO, not shown) through cannulation of the femoral artery and vein. b, Simplified schematic of the OrganEx perfusion device. The system is equipped with a centrifugal pump, pulse generator, haemodiafiltration, gas infusion, drug-delivery systems and sensors to measure metabolic and circulation parameters. c, Schematic of the experimental workflow and conditions. VF, ventricular fibrillation.