Extended Data Fig. 1 |. Analysis of circulation and blood/perfusate properties after 1h of warm ischaemia and perfusion interventions.

a, Representative fluoroscopy images of autologous blood flow (ECMO intervention, up) or a mixture of autologous blood and the perfusate (OrganEx intervention, below) in the head captured after 3 and 6 h respectively of perfusion, showing robust restoration of the circulation in the OrganEx group. A contrast catheter was placed in the left common carotid artery (CCA), except in the ECMO group at 6 h timepoint where contrast catheter could not be advanced beyond aortic arch in to the left CCA due to pronounced vasoconstriction, thus resulting in bilateral CCA filling. n = 6. b, Representative colour Doppler images of the CCA demonstrating robust flow in OrganEx group. Ultrasound waveform analysis demonstrated that OrganEx produced pulsatile, biphasic flow pattern (lower panel). SCM, sternocleidomastoid muscle; RI, resistive index. n = 6. c, Longitudinal change in arterial and venous cannula pressures throughout the perfusion demonstrating robust perfusion in OrganEx group. d, Time-dependent changes in oxygen delivery and consumption demonstrating increased oxygen delivery and stable oxygen consumption over the perfusion period in OrganEx group. n = 6. e, Presence of classical signs of death (rigor and livor mortis) in ECMO as compared to OrganEx group at the experimental endpoint. Data presented are mean ± s.e.m. Two-tailed unpaired t-test was performed. For more detailed information on statistics and reproducibility, see methods. *P < 0.05, **P < 0.01, ***P < 0.001.