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. 2022 Sep 16;18(9):e1010411. doi: 10.1371/journal.pgen.1010411

Fig 2. Effect of overexpression of miR-309a on ovarian development in B. dorsalis.

Fig 2

(A) Relative expression of miR-309a after the mimic injection. Data are means ± SE (error bars) of four biological replications. U6 as a reference gene was used to normalize the expression of miR-309a. (B) Surface area of ovary, including mimic-NC (n = 92) and mimic-309a (n = 93) treatments. Data are means ± SE. (C) Egg number. The fecundity of females in the mimic-309a treatment after successfully mating with untreated, naive males. Data are means ± SE of three biological replications. (D) Ovary phenotypes, including undeveloped ovaries, severe dysplasia of the ovaries, stunted ovaries without the formation of an ovarian tube, and stunted ovaries with a partially formed ovarian tube. The differences between means were analyzed by Student’s t test. For the significance test: *P < 0.05, **P < 0.01, ***P < 0.001.