Figure 6.

JAK1 overexpression partially reversed the inhibition of miR-194-5p on the malignant phenotype of GC cells.

(A) The expression of JAK1 protein in the transfected GC cells was monitored by Western blot. *** P < 0.001. (B) The proliferation ability of GC cells was assessed by CCK-8 assay. (C) EDU staining was used to reflect the proliferation ability of GC cells. (D) The invasion ability of GC cells was detected by transwell experiment. (E) The angiogenesis ability of GC cells was investigated by tube formation experiment. (F) The cancer stem cell characteristics of GC cells was evaluated by spheroidization experiment. *** P < 0.001 vs. miR-NC group. # P < 0.05 or ## P < 0.01 or ### P < 0.001 vs. miR-194-5p group.