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. 2022 Sep 28;8(39):eabo2954. doi: 10.1126/sciadv.abo2954

Fig. 3. Stereotactic injections of TAT-Cre/d(X)TAT cocktails into the brain of live mice achieve Cre-dependent recombination in vivo.

Fig. 3.

(A) Representative fluorescence images at ×2.5 magnification of brain slices of mice injected with TAT-Cre, with and without d(WW)TAT or d(LL)TAT. Injection of the AAV2-Cre is provided for comparison with state-of-the-art viral delivery technology. Inset: Bright-field image of the imaged brain slice. (B) Quantification of the tdTom fluorescence intensity in ×2.5 images. The results represent the average and corresponding SD of N = 3 independent injections (cropped images provided as examples, pseudocolored by fluorescence intensity). Measurements are made from the center of the area with the highest intensity in the direction perpendicular to the injection. a.u., arbitrary units.