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. 2022 Sep 28;8(39):eabo1123. doi: 10.1126/sciadv.abo1123

Fig. 6. Activation of HSF1 signaling plays a role in human KRAS-mutant LUAD cell growth.

Fig. 6.

(A) Representative images of crystal violet stained colonies formed by A-427 or SK-LU-1 cells treated with DTHIB or vehicle dimethyl sulfoxide (DMSO) 2 days after seeding, for 14 days. (B) Quantification of (A) from three biological replicates. Each condition was compared to controls that were plated in wells on the same plates. Bars represent means ± SD. *P < 0.05, **P < 0.01 by Student’s t test. (C) Proliferation of A-427 and SK-LU-1 cells treated with DTHIB or vehicle DMSO from day 4 after seeding. Representative of three independent biological replicates. (D) Representative images of crystal violet stained colonies formed by A-427 or SK-LU-1 cells upon genetic (shRNA) inhibition of HSF1. (E) Quantification of (D) from three biological replicates. Each condition was compared to shScramble controls that were plated in wells on the same plates. Bars represent means ± SD. ****P < 0.0001 by one-way ANOVA post hoc Bonferroni test. (F) Proliferation of A-427 and SK-LU-1 cells upon genetic (shRNA) inhibition of HSF1. Representative of three independent biological replicates.