Fig. 3. Postnatal epigenetic maturation in ARH neurons is associated with maturation of the response to dietary signals.

(A) GO biological function analysis of genes associated with common and sex-specific P35 > P12 neuron mDMRs. (B) Heatmap illustrating scaled enrichment of TF binding site motifs among neuron mDMRs that increase (P35 > P12) or decrease (P35 < P12) with age (left). K-means clustering (k = 2) divides these into two groups (top and bottom). Food deprivation–induced differential expression of TFs in AgRP neurons (right) (46). TFs regulated by food deprivation (FD) are enriched among the motifs associated with P35 < P12 mDMRs (χ² = 10.16, P < 0.01). (C) Scaled motif enrichment in male neuron P35 > P12 TOCs is highly correlated with that at mDMRs (P < 2.2 × 10⁻¹⁶).