TABLE 1.

Oligonucleotides used in this study

Primer	Sequence (5′-3′)a	Description
ON1	GGCCATGATATC ATTCTTGAAGACGAAAGGGCCTCG	EcoRV; 5′ end of ColE1 ori-bla
ON2	GGCCATGATATC CTGATATTGTCTGCATTTGCGCCG	EcoRV; 3′ end of ColE1 ori-bla
ON3	GGCCATATCGAT CCTGATATTGTCTGCATTTGCGCCG	ClaI; 5′ end of lacA-front
ON4	GGCCATATCGAT TCACAGTGGCAATCTCCCCCGTAT	ClaI; 3′ end of lacA-front
ON5	GGCCATATCGAT TTCAAGCTATATTTGGAGTTGAGC	ClaI; 5′ end of lacA-back
ON6	GGCCATCCCGGG CTAATGTGTGTTTACGACAATTCT	SmaI; 3′ end of lacA-back
ON7	GGCCATCCGCGG GAGCTC GGATCCCATTTCCCCCTTTGATTT	SacII and SacI; 5′ end of xylose cassette
ON8	GGCCATCCCGGG GAGCTC CTAACTTATAGGGGTAACACTTAA	SmaI and SacI; 3′ end of xylose cassette
ON9	GGCCATCCCGGG GACGTTCTTGCCATTGCTGCATAAA	SmaI; 5′ end of t0
ON10	GGCCATATCGAT ATCTCTGCAGTCGCGATGATTAAT	ClaI: 3′ end of t0
ON11	GGCCATATCGAT GCGGCCGC TCTAGAGCAACGTTCTTGCCATTG	SacII and NotI; 5′ end of erm
ON12	GGCCATCTCGAG GCGGCCGC ACTCTTCCTTTTTCAATATTATTG	ClaI and NotI; 3′ end of erm
ON13	GGCCATATCGAT TTGACATTTTTCTTGTGGATCTGTATAATAAAGAATAATTA	ClaI and −35 and −10 regions of a hybrid promoter; 5′ end of spec
ON14	GGCCATATCGAT CAATAGTTACAAATTGTTTCACTA	ClaI; 3′ end of spec
ON15	GGCCATGAGCTC AGGCCTTACACAGCCCAGTCCAGACTATTCGGCA	SacI; Plac expression cassette
ON16	GGCCATGAGCTC AGGCCTTAACTCACATTAATTGCGTTGCGCTCACTA	SacI; Plac expression cassette
ON17	GGCCATGGATCC ATGAATGTGTTATCCTCAATTTGTTAC	BamHI; 5′ end of bgaB
ON18	GGCCATGGATCC CTAAACCTTCCCGGCTTCATCATG	BamHI; 3′ end of bgaB
ON19	GGCCATGTCGAC CTAAATTTTAACTTAATTTATAATTAAACG	SalI; 5′ end of bgaB
ON20	GGCCATGCATGC CTAAACCTTCCCGGCTTCATCATG	SphI; 3′ end of bgaB
ON21	GGCCATGCATGC ATGTTAACAAATCGTCAGCTGCT	BamHI: 5′ end of hrcA
ON22	GGCCATGCATGC TGCCAAAATTCCCTTATTCATCATACAG	BamHI; 3′ end of hrcA

^aG/C clamps are shown in italics; restriction sites are underlined.