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. 2022 Sep 16;11:e67141. doi: 10.7554/eLife.67141

Figure 2. Cholesterol precursor 7-DHC and 7-DHC-derived Oxysterols are accumulated in Dhcr7-/- mouse embryonic cortex.

(A) Chemical structures of 7-DHC-derived oxysterols. LC-MS/MS analysis of (B) cholesterol and its precursors and (C) 7-DHC and cholesterol-derived oxysterols in Dhcr7+/+ and Dhcr7-/- embryonic cortex during development. Error bars indicate standard deviation. *, p<0.05; **, p<0.005; ***, p<0.001; n=3 biological replicates per group.

Figure 2.

Figure 2—figure supplement 1. Cholesterol biosynthesis pathway, the enzymes involved in the synthesis and the diseases associated with the defective enzymes.

Figure 2—figure supplement 1.

Related to Figure 2.
Figure 2—figure supplement 2. Cholesterol precursor 7-DHC and 7-DHC-derived Oxysterols are accumulated in SLOS-derived human iPSCs and NPCs.

Figure 2—figure supplement 2.

Related to Figure 2. SLOS patient derived hiPSCs and NPCs (3044 and 5788) along hiPSCs and NPC from healthy individual (emhf2) were cultured and used for LC-MS/MS analysis sterols and oxysterols. Quantitation of cholesterol, its precursors and accumulated 7-DHC derived oxysterols in SLOS patient derived hiPSCs and hiPSCs from healthy individuals (A) and in SLOS patient derived NPCs and NPCs from healthy individuals (B).