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. 2022 Sep 16;11:e67141. doi: 10.7554/eLife.67141

Figure 5. Dhcr7-/- mice display premature neurogenesis and increased activity of the TrkB neurogenic signaling pathway in vivo.

Figure 5.

(A) E18.5 cortical sections from Dhcr7+/+ and Dhcr7-/- were immunostained for Tbr1 (red), Ctip2 (green) and counterstained with DAPI (blue). (B and C) Quantifications of the absolute thickness (B) and the number of positive cells (C). (D–G) Cortical sections from E15.5 Dhcr7+/+ and Dhcr7-/- mice were immunostained for Satb2 (D, green) and Tbr1 (E, red). Quantifications of the absolute thickness (F) and the number of positive cells (G) for Satb2 and Tbr1. (H) E15.5 cortical sections from Dhcr7+/+ and Dhcr7-/- embryos EdU-labeled at E12.5 were immunostained for EdU (green) and counterstained with DAPI (blue). (I) Quantification of the relative location of EdU + cells in cortical sections. (J) E15.5 cortices were isolated from Dhcr7+/+ and Dhcr7-/- embryos and analyzed by western blot for phospho-TrkB, phospho-MEK, or phospho-C/EBPβ. Blots were re-probed with antibodies for total GR, TrkB, MEK, C/EBPβ and GAPDH as loading controls. (K) Quantification of phospho-TrkB, phospho-MEK, and phospho-C/EBPβ expression in E15.5 cortices were isolated from Dhcr7+/+ and Dhcr7-/- embryos. The relative levels of the phosphorylated proteins are normalized to GAPDH levels for each independent sample and expressed as fold increase. Error bars indicate SEM. *, p<0.05; **, p<0.005; ***, p<0.001. n=3 per experiment. Scale Bar = 50 μm.

Figure 5—source data 1. Related to Figure 5J.
E15.5 cortices were isolated from Dhcr7+/+ and Dhcr7-/- embryos and analyzed by western blot for phospho-TrkB, phospho-MEK, or phospho-C/EBPβ. Blots were re-probed with antibodies for total GR, TrkB, MEK, C/EBPβ, and GAPDH as loading controls.