Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | Adipose tissue progenitor cell | Corvera Laboratory | Mesenchymal progenitor cells | Cells used in this paper are generated from small fragments of surgically excised male or female human adipose tissue cultured in MatriGel, in the presence of normocin, penicillin and streptomycin. Cultures are recovered using dispase, expanded by two passages using trypsin, and frozen. Cells are then thawed and used for in experiments with no further passaging. Cells are not transformed, nor cultured beyond three passages and are therefore not routinely tested for mycoplasma nor subject to further authentication. Adipose tissue from which the primary cells are derived is obtained in accordance with the UMass Chan Institutional Review Board IRB ID 14734_13 |
| Antibody | Anti-Perilipin-1 (Rabbit monoclonal) |
Cell Signalling | #9349 | IF(1:500), |
| Antibody | Anti-MAOA (Rabbit monoclonal) | Cell Signalling | #73030 | IF(1:500) WB (1:500) |
| Antibody | Anti-MAOA (Rabbit monoclonal) |
Cell Signalling | #75330 | IF(1:500) WB (1:500) |
| Antibody | Anti-Tyrosine hydroxylase (Rabbit polyclonal) |
Millipore Sigma | #AB152 | IF (1:500) |
| Antibody | Anti-CD45 (Mouse monoclonal) |
Abcam | #282747 | IF (1:500) |
| Antibody | Anti-F4/80 (Rat monoclonal) |
Abcam | #6640 | IF (1:500) |
| Commercial assay, kit | Adiponectin ELISA | Invitrogen | #KHP0041 | Serum diluted 1:50 |
| Other | Isolectin GS-IB4 AlexaFluor-647 conjugate | Invitrogen | #I32450 | Staining, 1:200 |
| Other | Ulex Europaeus Agglutinin I DyLight 594 | Vector Laboratories | #DL-1067–1 | Staining, 1:200 |
| Other | DAPI stain | Invitrogen | #D1306 | Counterstaining 1 µg/mL |