FIGURE 3.

Commonly used configurations of transwell BBB models. Monoculture: BMECs are grown on the upper surface of the permeable support. Noncontact co-culture: BMECs are seeded on the upper surface of the support, while astrocytes (or other cell types) are seeded at the bottom of the culture well. Contact co-culture: BMECs are seeded on the top of the support and astrocytes (or other cell types) are seeded on the bottom of the support. Triple culture: In its most common form BMECs are seeded on the top of the support, pericytes seeded on the bottom surface, and astrocytes seeded on the bottom of the culture wells. BBB: Blood-brain barrier; BMECs: Brain microvascular endothelial cells.