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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2479:159–174. doi: 10.1007/978-1-0716-2233-9_11

Figure 2.

Figure 2.

Basic procedure for use of the Bacillus Genetic Stock Center deletion library. (A) Experimental outline of erm cassette removal to create clean deletions using pDR244, (B) pDR244 action inside the cell.