TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− (φ80ΔlacZΔM15) endA1 recA1 hsdR17 supE44 thi-1 λ− gyrA96 relAIΔ (lacZYA-argF)U169 | 26 |
| HB101 | F−hsdS20 (rB− mB−) Δ(gpt-proA) 62 leu supE44 λ− ara-14 galK2 lacY1 Δ (merC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13 | 5 |
| MT607 | pro-82 thi-1 hsdR17 supE44 rec A56 | 13 |
| S. meliloti | ||
| 1132 | Wild-type S. meliloti | 6 |
| 2011 | S. meliloti SU47 (RCR2011) | Rothamsted. Experimental station |
| 2011Rf-2 | Rif mutant of 2011 nod+ nif+; Rifr | This study |
| Plasmids | ||
| pBSL15 | 3.8-kb plasmid: Apr Kmr | 1 |
| pFD1001 | pRm1132f cloned into the PstI site of pSUP301; Kmr | 14 |
| pFD1192 | EcoRI/HindIII fragment of pRm1132f cloned into EcoRI/HindIII-digested pBR322 containing the oriT of pRK2; Tcr | 14 |
| pNRRM01 | pRm1132f cloned into the EcoRI site of pUC19, orientation +; Apr | This study |
| pNRRM2 | pRm1132f cloned into the EcoRI site of pUC19, orientation −; Apr | This study |
| pNRRa | pRm1132f amplified fragment (nt 2408–4501) cloned into SmaI-digested pBSL15, excised with PstI, and cloned into pSUP301; Kmr | This study |
| pNRSP1 | pRm1132f linearized with EcoRI and cloned into pSPT18 transcription vector; Apr | This study |
| pRK600 | pRK2013 npt::Tn9 Cmr Nm-Kms | 13 |
| pRm1132f | 7.2-kb plasmid from S. meliloti 1132 | 6 |
| pSPT18 | Transcription vector; Apr | Roche |
| pSUP301 | pACYC177 with oriT of RP4, ColE1 replicon; Apr Kmr | 24 |
| pSWEH | S1 nuclease-treated 2.4-kb EcoRI/HindIII fragment of pRm1132f and PstI-linearized pSUP301 followed by blunt-end ligation; Kmr | This study |
| pSWHC | S1 nuclease-treated 2.4-kb HindIII/ClaI fragment of pRm1132f and linearized PstI pSUP301 followed by blunt-end ligation; Kmr | This study |
a
nt, nucleotide.