TABLE 2.
Oligonucleotide primers for ORF-specific DNA
| ORFa | Primer | Nucleotide no. in plasmid sequence |
|---|---|---|
| ORF1 (F) | 5′-CCGAAAGCCTGACATATCG-3′ | 1242 |
| ORF1 (R) | 5′-GAGCGAACAGCCCTTTCTG-3′ | 1562 |
| ORF2 (F) | 5′-CATGCATGGGCGATCGACA-3′ | 2007 |
| ORF2 (R) | 5′-GCCAATCGACCACTACCTG-3′ | 2568 |
| ORF3 (F) | 5′-ATCGCGTGGGAAATGGCAGG-3′ | 3789 |
| ORF3 (R) | 5′-TTTCAGGCGGACCCTCTG-3′ | 4415 |
| ORF4 (F) | 5′-TATCGGGAGGCCATGAG-3′ | 5119 |
| ORF4 (R) | 5′-CTCGGGCACTGCGAATAT-3′ | 6059 |
| ORF5 (F) | 5′-CATCTCCAGAGCCAACGA-3′ | 4502 |
| ORF5 (R) | 5′-GTGCTGGTCCTTCCGCAC-3′ | 4783 |
| ORF7 (F) | 5′-ATTGACGGACCGAAGCGA-3′ | 1685 |
| ORF7 (R) | 5′-GGACGTCTGTCAGCGGAT-3′ | 2161 |
| ORF8 (F) | 5′-GACGAGCTCGGAACCGAA-3′ | 425 |
| ORF8 (R) | 5′-AACAGCTTGCAGCAGGCAC-3′ | 738 |
a
F, forward; R, reverse.