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Comparison of Novel Nested-Seq and RT-PCR methods for the analysis of SARS-CoV-2 mutations.
| Novel Nested-Seq | Wastewater SARS-CoV-2 RT-PCR variant panel (Promega Corp.) | |
|---|---|---|
| Sensitivity | High | High |
| Specificity | High | Low |
| Variants/mutations | Quantitative results | Qualitative results |
| Cost | High | Low |
| Labor | 1 week | 1 day |
| Data analysis | Bioinformatics/data interpretation | Not required |
| Prior knowledge of targets | No | Required |
| Number of targets | Unlimited | Defined based on the assay |
