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. 2022 Sep 14;13:935879. doi: 10.3389/fimmu.2022.935879

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Copyright © 2022 van der Pan, de Bruin-Versteeg, Damasceno, Hernández-Delgado, van der Sluijs-Gelling, van den Bossche, de Laat, Díez, Naber, Diks, Berkowska, de Mooij, Groenland, de Bie, Khatri, Kassem, de Jager, Louis, Almeida, van Gaans-van den Brink, Barkoff, He, Ferwerda, Versteegen, Berbers, Orfao, van Dongen and Teodosio

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Performance of the EuroFlow immunemonitoring innate myeloid cell (IMC) tube in a multicentric setting. (A) Principal component analysis (PCA) plot depicting the staining profile of 21 healthy adult peripheral blood samples processed at 4 centers and measured in 5 different instruments, employing the 11-color version (version 3) of the EuroFlow innate myeloid cell tube. Solid circles, represent the median values of the populations and the shades of color represent different centers and/or instruments. (B) depicts the intra-assay % of coefficient of variation for individual markers within centers (intra-center; biological variation) and between centers (inter-center, technical variation). cMo, classical monocytes; iMo, intermediate monocytes; ncMo, non-classical monocytes; pDC, plasmacytoid dendritic cells; myDC, myeloid dendritic cells; PC, principal component; CV, coefficient of variation; MFI PRP, Median Fluorescence Intensity of Positive Reference Population.