Table 1.

Antibody combinations used to stain peripheral blood for the selection of the best marker combination for identification of the different innate myeloid cell populations, and overview of the distinct versions evaluated during the multiple design cycles (four rounds) of the EuroFlow innate myeloid cell (IMC) flow cytometry tubes.

	BV421	OC515/BV510	BV605	BV650	BV711	BV786	FITC/BB515	PerCP Cy5.5	PE	PE CF594	PE Cy7	APC	AF700	APC H7/ APC C750	Samples evaluated (n=)
Backbone	–	CD45 OC515	CD62L	–	HLA-DR	CD16	–	CD36	Slan	–	–	CD300e	–	CD14 APC H7	–
pDC	–	CD45 OC515	–	–	HLA-DR	CD16	CD123 FITC	CD14	CD303	–	CD19	CD304	–	CD300e APC C750	5
	CD141	CD45 OC515	CD62L	–	HLA-DR	CD16	CD1c BB515	CD36	Slan	–	CD33	CD300e + CD304	–	CD14 APC H7	9
	CD141	CD45 OC515	CD62L	–	HLA-DR	CD16	CD1c BB515	CD36	Slan	–	CD33	CD300e + CD303	–	CD14 APC H7	4
myDC	CD141	CD5 BV510	CD19	CD11c	HLA-DR	CD16	CD1c BB515	–	–	–	CD33	CD300e + CD303	CD45	CD14 APC H7	5
Immature neutrophils	–	CD15 BV510	CD62L	–	HLA-DR	CD16	CD66b BB515	CD64	CD244	CD11b	CD33	CD300e	CD45	CD14 APCH7	5
M-MDSC	CD32	CD15 BV510	CD192	CD206	HLA-DR	CD16	–	CD36	CD124	CD11b	CD33	CD300e	CD45	CD14 APC H7	5
	CD32	CD15 BV510	CD192	CD206	HLA-DR	CD16	cyS100A9 FITC	CD36	CD124	CD11b	CD33	CD300e	CD45	CD14 APC H7	3
Axl+ DCs & preDCs	CD141	CD45 OC515	CD36	CD25	HLA-DR	CD33	CD100 FITC	CD1c	Axl	CD34	CD5	CD300e + CD303	CD16	CD14 APC H7	5
Version 1	CD141	CD45 OC515	CD62L	–	HLA-DR	CD16	CD1c BB515	CD36	Slan	–	CD33	CD300e + CD303	–	CD14 APC H7	11
Version 2	CD141	CD45 OC515	CD62L	–	HLA-DR	CD16	CD1c BB515	CD36	Slan + FcεRI	–	CD33	CD300e + CD303	–	CD14 APC H7	12
Version 3: 11-color version	CD141	–	CD62L	–	HLA-DR	CD16	CD1c BB515	CD36	Slan + FcεRI	–	CD33	CD300e + CD303	CD45	CD14 APC H7	121
Version 4: 14-color version	CD141	CD5 BV510	CD192	CD62L	HLA-DR	CD16	CD1c BB515	CD36	Slan + FcεRI	CD34	CD33	CD300e + CD303	CD45	CD14 APC H7	73

AF700, Alexa Fluor 700; APC, allophycocyanin; BB, Brilliant Blue; BV, Brilliant Violet, CF, Cyanin-based Fluorescent dye; Cy7, Cyanin7; FITC, Fluorescein isothiocyanate; H7, Hilite7; PE, Phycoerythrin; PerCP Cy5.5, Peridinin-chlorophyll-protein-cyanin 5.5; M-MDSC, Monocytic myeloid-derived suppressor cells; OC, Orange Cytognos; cy, cytoplasmic.