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. 2022 Sep 19;7(10):1568–1579. doi: 10.1038/s41564-022-01219-4

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© The Author(s) 2022, corrected publication 2024

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Fig. 5 — a, Schematics of PD-T4-9, PD-T4-10 and PD-λ-2 defence system operons and their domain predictions. Representative homologues of the systems are shown in their genomic contexts and indicate conservation and order of the system components. Blue, putative antitoxin; red, putative toxin; green, accessory factor. b,d,f,i, Each component or pair of components indicated was expressed (+) or not (−) from an inducible promoter and assayed for viable colony-forming units in tenfold serial dilutions. c,e, Plaquing assays for the phage indicated on cells harbouring an empty vector or a vector containing a given defence system with all components (WT) or lacking the component indicated. g,j, Plaquing of phages on TAC-containing strains expressing a second copy of the chaperone component to varying levels during infection. h, Schematic and T2 plaquing assay of the mqsRAC TAC system.