Extended Data Fig. 2. Neutralization of authentic wild-type virus by R1-32 compared to selected antibodies of different RBD targeting antibody classes.
a-g, Neutralization activities of wild-type virus (Wuhan strain) by a, R1-32 IgG, b, R1-32 Fab, c, B38 (ref. 59), d, rmAb23 (ref. 23), e, C144 (ref. 2), f, S309 (ref. 60), g, CR3022 (ref. 61) were evaluated by plaque reduction neutralization assays. Selected antibodies of different RBD antibody classes were included for comparison (see Extended Data Fig. 7 and Supplementary Fig. 3 for their classification and epitopes). Viruses were incubated with antibodies for standard 1 hr duration (black data points) or an extended duration of 16 hr (red data points) before inoculation (data points and error bars represent mean ± SEM). n = 3. Representative data from at least 2 independent experiments are shown. IC90 and IC50 values are summarized in the table below. Prolonged incubation improved R1-32 IgG neutralization likely due to more saturated R1-32 binding to spike.