Skip to main content
. 2022 Jul 6;127(7):1352–1364. doi: 10.1038/s41416-022-01867-7

Fig. 3. PFK158 works on the FAK-Stat3-SOX2 axis.

Fig. 3

Western blot analysis of molecular players contributing to PFK158 mediated stem cell population reduction in H28 and H2052 in dose-dependent (a), in a time-dependent manner in H28 (b) and PFKFB3-knockdown H28 clones with non-targeted control (c). Left: A decrease in Stat3-SOX2 interaction after PFK158 treatment as evident by immunoprecipitation with anti-Stat3 and subsequent immunoblotting with anti-Stat3 and anti-SOX2 in H226. Right: A bar graph of quantification of SOX2-Stat3 interaction normalised to the input (d) and (e). Left: H28 PFKFB3-knockdown clones sh55 and sh59 with its non-targeted control (NTC). Right: A bar graph of quantification of SOX2-Stat3 interaction normalised to the NTC2. Confocal microscopic images also revealed decreased interaction of Stat3 and SOX2 (f) after PFK158 treatment in an increasing manner for 24 h and (g) H28 knockdown clones. The lower panels of (f, g) show corrected total cell fluorescence (CTCF) of at least five different fields as mean ± SD. Images were taken in ×20 magnification.