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. 2022 Sep 28;12:16218. doi: 10.1038/s41598-022-19013-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Thrombopoietin and erythropoietin do not instruct MEP lineage commitment. (a) Index sorting and analysis of TPO receptor, c-Mpl (CD110). MEPs were index sorted into 96-well plates and grown in CFU assay conditions for 14 days. Colony type outcome was correlated with expression of CD110 at the time of sorting/plating. n = 66. Mean and SD indicated. (b) Representative colonies grown from individual MEPs in control, -TPO, and -EPO conditions. Mk/E mix, E-only, and Mk-only colonies were observed in control, -TPO, and -EPO CFU conditions at 14 days. Cells within the colony were stained in situ with CD41 (green), CD71 (magenta), and CD235a (red). Scale bar = 250 μm. (c) Frequency of colony types observed in control, -TPO, and -EPO CFU assays. Colony counts (Mk/E mix in blue, E-only in red, and Mk-only in green) were normalized to total colonies grown in control, -TPO, and -EPO conditions. n = 4. Mean and SD indicated. (d) Colony counts of MEPs grown in control, -TPO, and -EPO CFU assays. Colony counts (Mk/E mix in blue, E-only in red, and Mk-only in green) are reported out of 100 plated MEPs grown in control, -TPO, or -EPO CFU conditions. n = 4, *p < 0.05. Mean and SD indicated.