Table 4.

Summary of the regression data, the LOD, and the LLOQ of the targeted analytes

Substance	Calibration curve range (μg/mL)	Calibration curve	Weight	Coefficient of correlation	LOD (μg/mL)	LLOQ (μg/mL)
2,3-13C2 pyruvate	0.23–50	quadratic	1/x	0.9989	0.23	0.88
pyruvate	0.18–50	quadratic	1/x	0.9947	0.18	0.3
lactate	0.42–100	quadratic	1/x	0.9980	0.42	1.29
2-13C glycine	0.14–50	quadratic	1/x	0.9989	0.14	0.43
glycine	0.05–50	quadratic	1/x	0.9981	0.05	0.75
serine	0.34–50	quadratic	1/x	0.9982	0.34	0.45
1,2-13C2 glutamine	0.025–50	quadratic	1/x	0.9998	0.025	0.04
glutamine	0.11	quadratic	1/x	0.9994	0.11	0.2
1,2-13C2 glutamic acid	0.076–50	quadratic	1/x	0.9996	0.076	0.1
glutamic acid	0.22–50	quadratic	1/x	0.9995	0.22	0.5
1-13C AMP	0.04–50	quadratic	1/x	0.9993	0.04	0.05
AMP	0.028–50	quadratic	1/x	0.9986	0.028	0.045
acetyl CoA	0.07–50	linear	1/x	0.9984	0.07	0.085
ribose-5-phosphate	1–50	linear	1/x	0.9919	1	1.9
glyceraldehyde-3-phosphate	0.1–50	Linear	1/x	0.9995	0.1	1.2
fructose-6-phosphate	0.1–50	Linear	1/x	0.9939	0.1	0.2
phosphoglyceric acid	0.05–50	Linear	1/x	0.9978	0.05	0.08
ATP	0.025–50	Quadratic	1/x	0.9983	0.025	0.077
citric acid	0.1–20	Quadratic	1/x	0.9926	0.1	0.25