Table 5.

Intra-day and inter-day precision and accuracy for the targeted compounds

Precision
Substance	LLQC			LQC			MQC			HQC
	Conc	Intra-day	Inter-day	Conc	Intra-day	Inter-day	Conc	Intra-day	Inter-day	Conc	Intra-day	Inter-day
	μg/mL	CV%	CV%	μg/mL	CV%	CV%	μg/mL	CV%	CV%	μg/mL	CV%	CV%
2,3-13C2 pyruvate	1	3.9	4.1	1.5	2.9	4.9	10	2.2	3.2	50	3.9	6.3
pyruvate	0.3	0.6	2.1	0.7	0.6	0.4	10	0.9	2	50	1.5	7
lactate	1.5	3.5	2.1	3	6.2	7.9	50	1.4	4.6	100	1.7	6.5
2-13C glycine	0.4	2.9	5.4	0.6	4.5	9.6	10	1.7	6.7	50	3.9	10.7
glycine	0.75	0.2	0.4	1.5	0.3	0.3	10	5.0	6.0	50	2.1	12.2
serine	0.45	0.4	0.2	2	1.0	0.8	10	1.5	4.2	50	1.5	6.8
1,2-13C2 glutamine	0.04	5.0	1.8	0.055	1.8	5.1	10	3.2	4.2	50	2.2	10.9
glutamine	0.2	2.1	2.4	0.3	1.7	3.5	10	2.9	3.3	50	3.0	11.3
1,2-13C2 glutamic acid	0.1	5.5	7.0	0.14	7.0	10.7	10	3.9	9.8	50	4.2	12.6
glutamic acid	0.5	3.5	1.7	1	2.5	2.5	10	3.5	7.0	50	4.1	14.2
1-13C AMP	0.05	14.9	14.5	0.18	3.2	4.4	10	3.5	5.1	50	6.1	10.2
AMP	0.045	6.9	12.7	0.065	3.0	3.3	10	3.6	11.9	50	4.4	9.6
acetyl CoA	0.085	4.5	10.6	0.45	2.6	1.1	10	3.2	6.1	50	5.8	10.2
ribose-5-phosphate	1.9	1.4	1.2	2.8	5.4	1.8	10	9.2	6.8	50	7.8	7.3
glyceraldehyde-3-phosphate	1.2	8.2	7.6	1.5	9.4	3.6	10	5.0	7.4	50	3.1	6.4
fructose-6-phosphate	0.2	1.8	1.1	0.7	3.4	0.5	10	8.4	8.5	50	5.5	6.3
phosphoglyceric acid	0.08	0.6	0.6	0.1	2.2	2.8	10	2.6	6.8	50	2.9	7.2
ATP	0.08	11	7.9	0.25	6.4	7.1	10	4.2	4.0	50	5.3	10.6
citric acid	0.25	0.3	0.6	0.8	0.3	0.3	10	4.5	3.7	50	2.6	1.6

Accuracy
Substance	LLQC			LQC			MQC			HQC
	Conc	Intra-day	Inter-day	Conc	Intra-day	Inter-day	Conc	Intra-day	Inter-day	Conc	Intra-day	Inter-day
	μg/mL	RE%	RE%	μg/mL	RE%	RE%	μg/mL	RE%	RE%	μg/mL	RE%	RE%
2,3-13C2 pyruvate	1	2.4	3.9	1.5	−2.4	1.2	10	2.8	−1.6	50	2.3	−0.8
pyruvate	0.3	−1.4	−0.8	0.7	0.3	0.7	10	2.8	1.7	50	−1.4	−0.6
lactate	1.5	2.2	3.1	3.0	−13.1	−6.6	50	−4.0	−5.6	100	14.2	2.2
2-13C glycine	0.4	−6.4	−1.1	0.6	−6.1	10.9	10	3.0	−4.2	50	8.6	−5.3
glycine	0.75	0.1	0.2	1.5	0.0	0.2	10	7.7	−1.8	50	14.9	1.7
serine	0.45	0.2	0.1	2.0	1.5	1.1	10	2.6	2.6	50	1.4	1.4
1,2-13C2 glutamine	0.04	0.1	2.6	0.055	2.9	6.5	10	5.3	5.9	50	9.1	2.1
glutamine	0.2	−3.1	−1.1	0.3	2.4	4.1	10	5.2	5.5	50	7.6	0.2
1,2-13C2 glutamic acid	0.1	−2.3	−4.6	0.14	0.9	−2.0	10	3.7	2.1	50	5.4	2.9
glutamic acid	0.5	0.6	0.2	1	2.8	1.2	10	1.3	9.5	50	2.8	1.2
1-13C AMP	0.05	−5	6.2	0.18	0.0	−0.1	10	−1.0	4.4	50	−0.1	−0.6
AMP	0.045	13.2	−7.7	0.065	1.5	1.0	10	−2.2	−6.3	50	−4.1	−1.9
acetyl CoA	0.085	3.3	5.8	0.45	−0.2	1.0	10	1.7	−5.1	50	−3.2	−6.2
ribose-5-phosphate	1.9	1.6	1.1	2.8	8.4	1.1	10	0.1	−9.4	50	15.6	17.6
glyceraldehyde-3-phosphate	1.2	−1.7	−9.1	1.5	−0.1	2.2	10	−11	1.2	50	−0.8	−1.6
fructose-6-phosphate	0.2	0.6	2.0	0.7	−0.7	0.1	10	0.1	−7.6	50	2.6	4.9
phosphoglyceric acid	0.08	0.2	0.1	0.1	5.7	4.9	10	7.3	3.9	50	0.5	−2.9
ATP	0.08	−1.2	0.7	0.25	−0.7	−0.7	10	2.5	−1.9	50	15	8.0
citric acid	0.25	0.2	0.6	0.8	0.5	0.6	10	8.4	3.7	50	−0.4	1.2