Fig. 4.

T- and B-cell memory responses to commensal bacteria and intestinal fungi are dysregulated in CRC. A Heatmaps showing CD4/CD8 T-cell and B cell memory responses to indicated species, obtained from CellTrace Violet dilution data in gated populations of 7-day PBMC cultures with killed antigens. Each column shows results from one donor, with HC donors to the left and CRC donors to the right of the line. Numbers on scalebar represent % division. Hatched area: assays not performed. B Corresponding statistical analysis of data in A, showing overall p value for variance within each subset and comparisons of responses for each individual species below. Kruskal–Wallis ANOVA was used to analyze data with correction for multiple comparisons applied using the Benjamini, Krieger & Yekutieli method; n = 17 HC, n = 22 CRC