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. 2022 May 18;37(2):410–423. doi: 10.1007/s12028-022-01511-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Minocycline attenuated the interaction of activated microglia/macrophages and neurons in SAH-operated mice. Minocycline or vehicle was injected in SAH-operated mice, and brain cryosections were stained for neurons and microglia/macrophages. a Representative images of activated Iba1-positive cells (magenta) and NeuN-stained neuronal cell bodies (green) were recorded by confocal microscopy. The spatial interaction of these two cell types was assessed in images by counting the number of Iba1-positive cells contacting or overlapping with NeuN cells by the cell body at least one process. Nuclei were labeled with DAPI. White arrows point to microglia/macrophage interacting with neurons. b Magnification of a representative Iba1-positive cell in close vicinity of a neuronal cell. c The number of interactive microglia/macrophages related to the number of all activated microglia/macrophages was calculated. Values are means ± SEM (n = 6 animals per group, 4 brain slices per animal, and at least 4 images per brain). ***P < 0.001 versus SAH + vehicle. Statistical significance was determined by one-way ANOVA Bonferroni corrected. ANOVA analysis of variance, DAPI 4',6-diamidino-2-phenylindol, Iba1 ionized calcium-binding adapter molecule 1, SAH subarachnoid hemorrhage, SEM standard error of mean (Color figure online)