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MS survey scan followed by MS/MS DIA Scan events
| Multiplex ions | False |
|---|---|
| Collision energy mode | Stepped |
| Collision energy type | Normalized |
| HCD collision energies (%) | 25.5,27,30 |
| Orbitrap resolution | 30,000 |
| First mass | 200 |
| RF lens (%) | 40 |
| AGC target | Custom |
| Normalized AGC target (%) | 3,000 |
| Maximum injection time mode | Custom |
| Maximum injection time | 55 ms |
