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. 2022 Sep 23;90:106176. doi: 10.1016/j.ultsonch.2022.106176

Table 3.

Representative summary of previous works on enzymatic hydrolysis with ultrasound assistance.

Raw material Enzyme Enzyme dosage Sonication conditions Nanocellulose size (nm) Ref.
Cotton Celluclast 1.5 L 44
U/g fiber
20 kHz, 300 W, 90 min
(Simultaneous)
NA [205]
Cotton linter Cellulase
(Cerena sp.)
20
U/g fiber
30 kHz, 100 W, 20 min
(Post-treatment)
Z-avg = 204.6 ± 8.0 [206]
Eucalyptus kraft pulp Monocomponent endoglucanase 400
U/g pulp
20 kHz, 315 W, 10 min
(Post-treatment)
W = 6–10
L = 400.0 – 600.0
[196]
Cotton Cellulase

(Aspergillus Niger)
<100 µg/mL NA
(Post-treatment)
W = 30–45
L = 250–900
[207]
Microcrystalline cellulose Endoglucanase
(Penicillium oxalicum M12)
15
mg/g
40 kHz, 100 W, 30 min
(Post-treatment)
W = 17.9–33.8
Z-avg = 357–863
[208]
Poplar wood Celulase
(Novozyme)
10
U/g wood
25 kHz, 200 W, 20 min
(Post-treatment)
Z-avg = 310.0 ± 5.0 [209]
W, width; L, length; Z-avg, Z-average.