Fig. 10. Intravenous injection of the AAV8-TBG-siR^T+B+I induces long-term combination therapy in the DSS-induced chronic UC model.

a Flow chart of the experimental design. b Evaluation of AAV-mediated luciferase expression to reflect co-expressed siRNA accumulation in vivo (n = 6 in each group). c Body weight curves (n = 6 in each group). d DAI scores (n = 6 in each group). e Representative macroscopic features of colons. Scale bar: 1 cm. f Representative images of H&E staining of colon sections. Scale bar: 100 μm. Each H&E staining was repeated independently three times, and representative images are shown. g–i Quantitative RT-PCR analysis of the absolute expression levels of TNF-α siRNA, B7-1 siRNA and integrin α4 siRNA in the colon (n = 5–6 in each group). j Determination of the absolute expression levels of TNF-α protein in the colon by ELISA (n = 6 in each group). k The population of B7-1⁺ cells in total colonic lamina propria mononuclear cells (n = 2–3 in each group). l The population of integrin α4⁺ lymphocytes in total colonic lamina propria lymphocytes (n = 3–4 in each group). Values are presented as the mean ± SEM. Significance was determined using one-way ANOVA followed by Dunnett’s multiple comparison in (d, j, k, l) or using two-way ANOVA followed by Dunnett’s multiple comparison in (c). *p < 0.05; **p < 0.01; ***p < 0.005.