Table 4.

Methods for the diagnosis of human Flavivirus infections.

Methods	Advantages	Limitations	References
Virus isolation	Direct pathogen detection Most specific and conclusive diagnosis	Time consuming Laborious Requirement of acute sample Biosafety Laboratory considerations of level 2–4	(Goncalves et al., 2017) (Musso and Desprès, 2020) (Samuel Sulca Herencia, 2019)
RT-PCR	Detection of viral nuclei acids High sensitivity and specificity Rapidity	Require careful handling to prevent cross-contamination Require specialized instrumentation Expensive
Viral antigen capture	Detection of acute of DENV based on the capture of soluble NS1 Easy to perform	Only available for DENV Less accurate than viral isolation Requirement of acute sample
Serology	Detection through the capture of IgG/IgM or virus neutralization assays Qualitative and quantitative serological diagnosis tests	Limited specificity and sensitivity False interpretation of DENV diagnostic might occur during the secondary DENV infection