Fig. 2. Three candidate RBPs were capable of binding exon 30 and adjacent intronic areas of ITSN1 RNA.

A Schematic representation of the two isoforms of human ITSN1 resulting from an alternative splicing. B Schematic diagram of RNA pull-down assay and proteomics approaches. C In vitro RNA pull-down assay. Proteins were subjected to SDS-PAGE and silver stained after pull-down. D The top 20 RBPs ranked by MS score of RNA group. E The Venn diagram showed 25 overlapped RBPs between 184 RNA pull-downed RBPs (MS score >100) and 94 RBPs with known binding motifs. F, G Circos plots showed the differential expression analysis of the 25 overlapped RBPs in LGG (F) or GBM samples (G) and their paired normal samples from GEPIA (cut-off: |log₂FC| > 1, q-value < 0.01). H A box plot showed the expression profile of the overlapped RBPs across non-neoplastic samples and grade II-IV glioma samples.