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. 2022 Sep 28;13(9):835. doi: 10.1038/s41419-022-05238-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A PTBP1 expression was evaluated between LGG or GBM and normal samples by GEPIA. B Scatter plot comparing PTBP1 mRNA levels between different grades of glioma using the data from CGGA. C Curves of Kaplan–Meier analysis. OS was analyzed according to the PTBP1 expression (cut-off at 50% of the entire group). Low PTBP1 expression was related to long OS (P < 0.001). D Western blot analysis of PTBP1 knockdown and rescue in the extracts of cells as indicated. Loading control: β-actin. E Images of EdU staining (left, scale bars, 100 μm) and the comparison of EdU-positive rates between the control and silencing PTBP1 cells (right). F Migration assay of the indicated cells. Cells migrating through transwell inserts were stained, photographed, and quantified. Scale bars, 200 μm. G Invasion assay results. Cells invading through matrigel matrix-coated transwell inserts were stained, photographed, and quantified. Scale bars, 200 μm. Values were expressed as mean ± SD from three independent experiments in (E–G) (two-tailed Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001). H Nude mice injected with control or silencing PTBP1 LN229 cells. The images shown represent results for 7 mice. Red and blue dotted lines indicated the location and size of the tumors. I Growth curve of nude mice tumors within 7 weeks, values were expressed as mean ± SD (two-way ANOVA, ***P < 0.001). J The representative images show the xenograft tumors isolated from nude mice at the 7th week of tumor inoculation. K Representative images of hematoxylin-eosin staining (left), scale bars, 200 μm. The frequency of xenograft invasion in mice was analyzed quantitatively (right), P value was calculated using the chi-square test. ***P < 0.001. L Immunohistochemical images of tumor sections stained for Ki67 from control or PTBP1 knock down mice (left), quantitation of the percentage of Ki67-positive cells in tumor sections (right). Values are mean ± SD, two-tailed Student’s t test, ***P < 0.001. Scale bars, 200 μm.