FIG. 5.

Relative rates of adenylation of the full-length ligase and the 40-kDa N- and 30-kDa C-terminal domains. A series of assays containing a 1.0 μM concentration of protein and 0.01 μM [³²P]NAD⁺ were incubated at room temperature and terminated at selected time points by the addition of SDS to a final concentration of 3.3%. Phosphorimaging was used to quantify the percentage of enzyme adenylated with labeled NAD⁺.