Skip to main content
. 2022 Aug 28;298(10):102428. doi: 10.1016/j.jbc.2022.102428

Figure 3.

Figure 3

MBD2sc becomes static at the methylated CpG-rich regions on DNA tightropes.A, a cartoon drawing of the CpG-free–rich DNA fragment (top panel) and ligated DNA substrate containing alternating mCpG-rich and CpG-free sequences (mCpG-free–rich, bottom panel) for the DNA tightrope assay. B, kymograph of MBD2sc on the ligated mCpG-free–rich DNA tightrope. C, Histogram of the distance between adjacent MBD2sc-QDs on mCpG-free–rich DNA tightropes. The solid lines represent Gaussian fit to the data (R2> 0.98) with a peak centered at 2.2 (±0.3 μm) (N = 461, the error bars represent mean ± SD). MBD, methyl-CpG–binding domain; CpG, cytosine-guanosine dinucleotide; mCpG, methylated CpG.