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DNA tightrope assay of MBD3sc on unmethylated and methylated DNA substrates.A, representative kymographs of MBD3sc on different DNA tightropes. B and C, diffusion coefficients (B) and alpha exponents (C) of MDB3sc on CpG-free, CpG-free–rich, and mCpG-free–rich DNA tightropes. ∗: p < 0.05; ∗∗∗: p < 0.001. See Table 1 for detailed analysis. MBD, methyl-CpG–binding domain; CpG, cytosine-guanosine dinucleotide; mCpG, methylated CpG.
