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Experimental timeline. All animals underwent repeated cycles of alcohol consumption (in a free choice paradigm with water, 5, 10, or 20% ethanol solutions) and deprivation phases (only water available). After the sixth alcohol deprivation phase pharmacological intervention experiments were conducted. The number eight and four refers to 8 and 4 weeks of alcohol drinking, respectively. The number two stands for a 2 weeks deprivation period.
