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Growth of plasmid-carrying strains
| Strain | Plasmid carried | Genotype | Growth ata:
|
||
|---|---|---|---|---|---|
| 30°C | 42°C | 42°C plus oleate | |||
| DM86 | pAG58 (control) | fabB(Ts) | + | − | + |
| DM86 | pGES20 (fabFb) | fabB(Ts) | + | − | + |
| CY288 | pAG58 (control) | fabB(Ts) fabF | + | − | − |
| CY288 | pGES20 (fabFb) | fabB(Ts) fabF | + | − | + |
Growth was scored by the appearance of single colonies on solid LB medium. Oleate (18:1) when added was present as the potassium salt at a concentration of 100 μg/ml. −, no growth; +, growth.
