Fig. 3.

The cell proliferation and cellular uptake of selected LDH nanoparticles in hUC-MSCs. A) CCK8 analysis of MgAl–Cl and MgFe–NO₃ LDH treated hUC-MSCs for 1 day and 3 days under different concentrations (1, 2.5, 5, 10, 20 and 40 μg/mL). *p < 0.05, ***p < 0.001. B) Flow cytometry analysis of cellular uptake of MgAl–Cl and MgFe–NO₃ LDH in hUC-MSCs after different time incubation (0.5, 1, 2, 4, 6, 8, 24 and 48 h). C) Metal ion release of MgAl–Cl LDH and MgFe–NO₃ LDH in PBS after different time. D) Immunofluoresence of cellular uptake of MgAl–Cl and MgFe–NO₃ LDH conjugated with FITC in hUC-MSCs after 24 h incubation, the organelle was labeled with mito-red (mitochondrial labeling, up) and lyso-red (lysosome labeling, down), respectively. Scale bar = 10 μm. E) Mitochondrial membrane potential of MgAl–Cl LDH and MgFe–NO₃ LDH treated hUC-MSCs for 1 day and 3 days. Scale bar = 100 μm.