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. 2022 Sep 26;22:75–90. doi: 10.1016/j.bioactmat.2022.08.023

Fig. 4.

Fig. 4

The in situ IVD regeneration function of optimizing LDH pretreated hUC-MSCs for 7 days chondrogenic differentiation and transplantation in the injury site of rat model. A) The schematic diagram of IDD model constructed by needle puncture. B) The photo records of surgical model construction. C) X-ray analysis of IVD height change with different treatment after 4 weeks. D) X-ray analysis after 8 weeks. E) MRI analysis after 8 weeks. F) MRI analysis after 12 weeks. G) The disc height index (DHI) of optimizing LDH-pretreated cell transplantation effects 4 weeks and 8 weeks after surgery. *p < 0.05, **p < 0.01, ***p < 0.001. H) H&E and S&O staining of different treatment after 12 weeks. I) Immunofluorescent of COL1A1, COL2A1 and Ki67 staining of different treatment after 12 weeks. White arrows indicate positive cells. Scale bar = 100 μm. J) Tissue H&E staining of main organs including heart, liver, spleen, lung and kidney in Sham, Control, Cell and LDH groups after 12 weeks operation. Scale bar = 500 μm.