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. 2022 Sep 26;22:75–90. doi: 10.1016/j.bioactmat.2022.08.023

Fig. 7.

Fig. 7

Focal adhesion signaling pathway validation of optimizing LDH regulated hUC-MSCs toward chondrogenic differentiation. A) qPCR analysis of focal adhesion signaling pathways markers Integrin, Vinculin and Cdc42 under different concentrations of integrin inhibitor ATN (5, 10, 20, 50 and 100 μM, respectively). **p < 0.01, ***p < 0.001. Scale bar = 100 μm. B) Cell migration detect of LDH, ATN and LDH + ATN groups. C) The migration index of figure B quantified by image J. ***p < 0.001. D) Immunofluorescence of LDH, ATN and LDH + ATN treated hUC-MSCs for 7 days chondrogenic differentiation. Scale bar = 25 μm. E) qPCR analysis of chondrogenic markers SOX9, COLX, COL3A1 and COL6A1 expression. *p < 0.05, ***p < 0.001. F) Western blot analysis of focal adhesion related proteins after the treatment of LDH, ATN and LDH + ATN for 7 days chondrogenic differentiation.