Skip to main content
. 2022 Sep 24;57:102491. doi: 10.1016/j.redox.2022.102491

Fig. 1.

Fig. 1

Depiction of the experimental design and levels of ascorbate in the liver of mice. (A) Males and females were separated into six experimental groups. Each group contained three males and three females. Animals were labeled according to their genotype (GL for Gulo−/− mice and WT for wild-type mice) and the vitamin C (VitC) treatments (% is weight of ascorbate per 100 mL of drinking water). The vitamin C (or ascorbate) treatment sessions for each group are depicted on the top of each timeline. The liver was harvested at the age of 4 months for all mice. (B) Histogram showing the ascorbate levels in the liver of each indicated female cohorts (N = 3 females per group). * = significantly different compared to FWT00, FGL40, and FGLR40 (with a Tukey post ANOVA tests p-value < 0.05). † = significantly different compared to FGL00 and FGL01 (with a Tukey post ANOVA tests p-value < 0.05). (C) Histogram showing the ascorbate levels in the liver of each indicated male cohorts (N = 3 males per group). * = significantly different compared to MWT00, MGL40, and MGLR40 (with a Tukey post ANOVA tests p-value < 0.05). (D) Histogram comparing liver ascorbate levels between the different groups of males and females receiving the same ascorbate treatments. Bars in each histogram represent mean ± SEM. Significant differences are indicated using * (with a Tukey post ANOVA tests p-value < 0.05).