Fig. 2.

Reproducibility of the liver microsomal enrichment extraction procedure on the different groups of mice. (A) Schematic representation of the different steps undertaken to obtain different cellular fractions. (B) Example of western blots showing protein levels of IRE1α, Grp78, calreticulin, Sod2, Aif, catalase, Svct1, Hsc70, and topoisomerase I (Topo-I) in the different liver fractions. Each lane contains 15 μg of proteins. (H = whole cell homogenate; P = pellet fraction from the first step of the procedure; S = supernatant fraction of the last step of the procedure; MEE microsomal enriched extract). (C) Gene ontology analysis of the proteins identified by mass spectrometry showing the enriched cellular compartments after the MEE procedure.