Fig. 6.

Gene Ontology and protein network analyses showing the biological processes correlating with liver ascorbate levels in both males and females. (A) Biological processes analyses of proteins that correlated with liver ascorbate levels in both female and male mice using DAVID. Biological processes with Bonferroni p-value < 0.05 are presented in the histogram. (B) Protein-protein interaction networks of common proteins in females and males correlating positively with liver ascorbate levels using STRING. Colored nodes: green = carbohydrates and fatty acids metabolisms (FDR = 1.52 × 10⁻⁷); red = mitochondrial complex III of the electron transport chain (FDR = 7.29 × 10^–13); blue = iron homeostasis (FDR = 0.0343); gray = hepatic sulfotransferase involved in the maintenance of bile acid homeostasis. (C) Protein-protein interaction networks of common proteins in female and males correlating negatively with liver ascorbate levels using STRING. Colored nodes: red = classical complement activation pathway (FDR = 0.0010); blue = positive regulation of immune response (FDR = 0.0055); gray = associated with the extracellular matrix. The protein nomenclatures are depicted in the supporting information Table 6. The thick edges represent physical interactions. The thin edge represents co-expression in the same metabolic pathway. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)