Fig. 7.

Immunoblot analyses in ascorbate deficient Gulo^−/− mice livers validating the differential expression of mitochondrial complex III proteins. Analyses of six different proteins from the mitochondrial electron transfer chain complexes by western blots on whole liver lysates for females (A) or males (B). Signal quantification of three proteins from the complex III over total protein staining: Uqcrc1 (C), Uqcrc2 (D), Uqcrfs1 (E). (F) Ratio between the Ndufβ8 protein signal from the complex I and total protein staining. (G) Ratio between the Sdhb protein signal from the complex II and total protein staining. (H) Ratio between the Atp5α protein signal from the complex V and total protein staining. (I) Ratio between the β-actin protein signal and total protein staining. (J) Coomassie-brilliant-blue staining quantification. Bars in each histogram represent mean ± SEM (N = 4 females and males combined per group). Significant differences are indicated using * (with a Tukey post ANOVA tests p-value < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)