TABLE 1.
Convergence of 18-month-old NTg and 3xTg-AD females’ phenotypes in the corner test, open field test and object recognition test.
| NTg mice (n = 9) | 3xTg-AD (n = 8) | 3xTg-AD-ISO (n = 9) | ||||
| Mean ± SEM | Mean ± SEM | G | Mean ± SEM | G | ISO | |
| Corner test (30 s) | ||||||
| Visited corners (n) | 7.22 ± 0.88 | 5.88 ± 1.27 | n.s | 5.56 ± 0.85 | n.s | n.s |
| Rearing (latency, s) | 17.89 ± 3.02 | 16.63 ± 3.81 | n.s | 13.11 ± 3.32 | n.s | n.s |
| Rearings (number) | 1.44 ± 0.29 | 1.88 ± 0.58 | n.s | 2.56 ± 0.41 | n.s | n.s |
| Open field test (Day 1, 5 min) | ||||||
| Initial freezing (latency, s) | 20.56 ± 13.79 | 3.00 ± 0.63 | n.s | 5.00 ± 0.87 | n.s | n.s |
| Exit of the center (latency, s) | 28.33 ± 13.98 | 9.75 ± 1.94 | n.s | 44.78 ± 31.96 | n.s | n.s |
| Entrance to the periphery (latency, s) | 78.33 ± 37.12 | 64.75 ± 31.60 | n.s | 94.33 ± 41.35 | n.s | n.s |
| Vertical activity (latency, s) | 161.78 ± 38.45 | 175.38 ± 41.39 | n.s | 210.22 ± 33.49 | n.s | n.s |
| Bizarre, vertical rearing (latency, s) | 265.33 ± 26.1 | 207.88 ± 42.5 | n.s | 295.89 ± 4.1 | n.s | n.s |
| Self-grooming (latency, s) | 161.00 ± 26.49 | 147.50 ± 27.36 | n.s | 161.33 ± 28.44 | n.s | n.s |
| Self-grooming (n) | 1.33 ± 0.29 | 1.25 ± 0.25 | n.s | 1.00 ± 0.24 | n.s | n.s |
| Defecation boli (n) | 2.78 ± 0.46 | 3.63 ± 0.84 | n.s | 3.00 ± 0.50 | n.s | n.s |
| Context and object recognition test Open field test (Day 2, 1 min) | ||||||
| Initial freezing (latency, s) | 20.11 ± 8.12 | 9.71 ± 2.53 | n.s | 7.00 ± 2.15 | n.s | n.s |
| Exit of the center (latency, s) | 23.44 ± 7.80 | 17.29 ± 3.99 | n.s | 13.38 ± 4.39 | n.s | n.s |
| Entrance to the periphery (latency, s) | 23.44 ± 8.52 | 24.57 ± 6.22 | n.s | 23.50 ± 7.77 | n.s | n.s |
| Vertical activity (latency, s) | 50.67 ± 6.72 | 53.86 ± 4.09 | n.s | 40.50 ± 7.62 | n.s | n.s |
| Bizarre, vertical rearing (latency, s) | 60.00 ± 0.00 | 60.00 ± 0.00 | n.s | 60.00 ± 0.00 | n.s | n.s |
| Self-grooming (latency, s) | 56.67 ± 3.33 | 60.00 ± 0.00 | n.s | 59.00 ± 0.94 | n.s | n.s |
| Self-grooming (n) | 0.33 ± 0.17 | 0.00 ± 0.00 | n.s | 0.14 ± 0.13 | n.s | n.s |
| Defecation boli (n) | 1.22 ± 0.46 | 0.29 ± 0.17 | n.s | 0.14 ± 0.13 | n.s | n.s |
| Object recognition test (Day 2, 10 min) | ||||||
| Latency 1st object (s) | 142.67 ± 76.52 | 60.29 ± 42.74 | n.s | 60.13 ± 19.48 | n.s | n.s |
| Latency 2nd object (s) | 235.00 ± 65.45 | 362.57 ± 85.02 | n.s | 238.00 ± 80.77 | n.s | n.s |
| Time 1, acquisition criteria (s) | 551.00 ± 40.01 | 490.71 ± 50.53 | n.s | 408.88 ± 73.64 | n.s | n.s |
| Total touch duration (s) | 10.50 ± 2.58 | 12.14 ± 2.95 | n.s | 14.12 ± 2.35 | n.s | n.s |
| Animals to reach acquisition criteria | 1/9 | 3/8 | n.s | 4/9 | n.s | n.s |
Statistics: Results are expressed as mean ± SEM or incidence. Student t-test, G, genotype effect (vs. NTg mice), ISO, isolation effect in 3xTg-AD mice (vs. 3xTg-AD mice housed in groups). All n.s., p > 0.05.