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| Sample No | Reagents/Instruments | Penalty points |
|---|---|---|
| 1 | Acetate buffer | 0 |
| 2 | Acetonitrile | 4 |
| 3 | Copper acetate | 1 |
| 4 | Acetic acid | 2 |
| 5 | Tetra butyl ammonium hydroxide 10% in water | 3 |
| 6 | HPLC | 1 |
| 7 | Occupational hazard | 0 |
| 8 | waste | 5 |
| 9 | Total penalty points | 16 |
| 10 | Analytical Eco-Scale | 84 |
