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. 2022 Sep 15;13:1007202. doi: 10.3389/fimmu.2022.1007202

Table 1.

Program for the vaccination experiment.

Group Strains for immunization Method ofAdministration Dose Time ofAdministration
Ctrl Blank control Blank control Blank control Blank control
168-spores/mixed feeding B. subtilis 168 Fed the mixed diet 2.5×106 CFU/g feed The whole period
RB-spores/gavage B. subtilis RB Gavage 2.0×1010CFU/mL* Days 1-3, 14-16, and 28-30
RB-spores/mixed feeding B. subtilis RB Fed the mixed diet 2.5×106 CFU/g feed The whole period
Inactivated vaccine Commercial vaccine Intraperitoneal injection 100μL/per mouse Days 1, 14, and 28

* The bacterial concentration was adjusted to 2.0×1010CFU/mL with normal saline. On day 1-3, 14-16, and 28-30, each mouse was given 0.1 mL intragastric administration every day for 3 consecutive days.