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No significant effect of temperature on kissing loop interactions. (A) Example ionic current traces for 100 nM EM measured at 200 mV and at different temperatures. (B) Scatterplots as a function of voltage measured at 23 °C. There is no appreciable change in blockade ratio upon increasing the temperature up to 60 °C (see SI: Fig. S8); only dwell time becomes faster, suggesting that the kissing loop interactions in these fiber structures are resistant to heating at this temperature.
