Figure 5.

A MITE insertion in the MdRFNR1-1 promoter is associated with its allelic induction by simulated drought stress. A, Allelic expression of MdRFNR1 in GL-3 under control and PEG treatment. Error bars indicate sd, n = 3. B, GUS activity of transgenic calli carrying MdRFNR1-1_pro:GUS or MdRFNR1-$1_{\text{pro}}^{\Delta \text{MITE}}$:GUS under control and PEG treatment. Error bars indicate sd, n = 4. C, GUS staining of Arabidopsis plants carrying MdRFNR1-1_pro:GUS or MdRFNR1-$1_{\text{pro}}^{\Delta \text{MITE}}$:GUS in response to PEG treatment. Bars = 4 mm. Statistical analyses were performed by the Student’s t test. Statistically significant differences are indicated by **P ≤ 0.01 or ***P ≤ 0.001, ns, no significant difference.